In Super Punch-Out!! the player controls Little Mac, as he fights his way to become the World Video Boxing Association champion. Players, fighting from a "behind the back" perspective, must knockout their opponent in three minutes to win. Players can launch jabs, hooks, and uppercuts against their opponents as well as block, dodge, and duck opponents' attacks.

Face off against old favorites including Bear Hugger, Piston Hurricane, Bald Bull, Mr. Sandman and Super Macho Man plus a cast of new and weird challengers. With a right hook! Body blow! And precise quick jabs! The referee announces "Knock Out!" Your opponent lies face down as you win the title of Nintendo Video Boxing Association Champion!