TwinBee is a vertical scrolling shooter. The players control cartoon-like spacecrafts with anthropomorphic arms, player one being TwinBee and player two being WinBee. The spacecrafts can be moved in all 8 directions, with one button for regular shooting flying enemies and one button for dropping ground bombs.

In the two-player mode, TwinBee (cyan craft) and WinBee (red craft) have the same speed and they can join "hand-to-hand" and fire a super blast. Also in two player mode, if one craft is hit by the other from behind, it will fire four multi-directional shots.