Tiny Toon Adventures: Acme All-Stars features all the usual characters from the Tiny Toon Adventures animated series.

There are several different game options. The main two games are Soccer and Basketball. Both of these options allow you to pick your own team of 4 characters and to choose their playing positions. You can then pick from 5 different sporting arenas. Play against the CPU or against a friend. The Story Mode sees a predetermined team competing at several different events at the Acme Looniversity Tournament.